Journal of Stem Cell Research & Therapy

The characterization of variant transcripts of oncogenes is necessary for cancer cell therapy

3^rd International Conference and Exhibition on Cell & Gene Therapy

October 27-29, 2014 Embassy Suites Las Vegas, USA

Mojtaba Darbouy

Accepted Abstracts: J Stem Cell Res Ther

Abstract:

The alternative splicing usage is now recognized as a common mechanism in the transcriptional regulation of gene to generate variant transcripts. The variant transcripts obtained from a single gene could be generated by a single promoter or multiple promoters which contribute to protein isoforms or a single protein respectively. An oncogene is a kind of abnormal gene that predisposes cells to develop into cancer which helps drive the uncontrolled growth that underlies tumor. The cancer cell is established by oncogene in that the over expression is an obligatory way to initiate normal cell to cancer cell. It has been shown that there about forty genes which could act as an oncogene. All of these genes have alternative splicing from which the variant transcripts have been produced. Moreover, the cancer cells express a broad collection of splice variants that may be distinct from those occurring in normal cell. The divers transcripts obtained from the oncogene have a cellular specific expression that defined a particular state of the cell. The characterization of splice variant transcripts in cancer cell versus normal cell could be a target for the strategy design of therapy approach such as: Antisense oligonucleotide, microRNA and recombinant monoclonal antibody against the resulting proteins. This therapy might be useful for down regulation of oncogene expression or blocking the function of resulting proteins.