Sana Dibazar and Leila Golpasand Hagh
Ahvaz Jundishapur University of Medical Science, Iran
Posters & Accepted Abstracts: Oral Health Dent Manag
Statement of the Problem: Recently, there has been an increasing growth in research on medical plant???s effect on
dental plaque bacteria.
Aim: The aim of this study was to determine the antibacterial effects of Satureja hortensis extract and its essential
oil (EO) on Streptococcus salivarius, Streptococcus sanguis and Streptococcus mutans as important bacteria in early
supragingival dental plaque formation.
Methodology & Theoretical Orientation: In this in vitro study, different concentrations of S. hortensis extract and
its EO were prepared using double dilution method. The disc diffusion method was used to determine antibacterial
activity. Based on these measurements, the minimal inhibitory concentration value was reported for each bacterium.
Antibiotics used as positive controls in this study were erythromycin (15 ?g) and tetracycline (30 ?g). T-test and
ANOVA were used for statistical analysis (P<0.05).
Findings: Aqueous and methanolic extract did not show significant antibacterial activity, but the EO significantly
inhibited the growth of the test bacteria compared to positive control (P<0.05). For S. mutans, the inhibition effect of
tetracycline 30 ?g was similar with 50% (P=0.789) and 25% (P=0.158) dosages of the EO. For S. salivarius, the effect
of tetracycline 30 ?g was similar to 50% dosages of the EO (P=0.122). For S. sanguis, the effect of erythromycin 15 ?g
was lower than 50% (P=0.0006) and 25% (P=0.003) dosages of the EO. The inhibition effects of all concentrations of
EO were higher for S. sanguis, S. salivarius and S. Sanguis are more sensitive than S. mutans to S. hortensis EO. So,
due to the strong antibacterial effect of S. hortensis EO on the oral bacteria growth, it can be served as herbal mouth
rinse, while to confirm this antibacterial effect, further clinical studies are necessary.
E-mail: sana.dibazar@yahoo.com