Brain Disorders & Therapy

Targeting proteoglycan receptor PTP?? promotes regeneration of sensory axons after spinal cord dorsal roots injury

7^th International Conference on Neurological Disorders & Stroke

September 20-21, 2018 | Rome, Italy

Min Yao, Haitao Sun, Gilberto K K Leung and Wutian Wu

The University of Hong Kong, Hong Kong
Southern Medical University, Guangdong, P R China

Scientific Tracks Abstracts: Brain Disord Ther

Abstract:

Traumatic injury to the spinal dorsal roots usually always results in permanent sensory deficits. The inhibitory barriers at dorsal root entry zone (DREZ) could prevent both axonal entry and regeneration in the spinal cord. CSPGs are dominant suppressors in DREZ and exert inhibition via neuronal receptors including PTP??. ISP (Intracellular Sigma Peptide) is a small peptide memetic of the PTP?? wedge region, it could target PTP?? to relieve inhibition of CSPG. Adult female Sprague Dawley rats were performed to unilateral cervical 5 to thoracic 1 dorsal roots crush, ISP or vehicle was daily administrated with subcutaneous injection near the injury site. Our results show that ISP could significant increase sensory axons regeneration into spinal cord without abnormal regrowth on intact sides (Fig.1, Fig.2). Also, ISP promoted primary sensory fibers regeneration across the DREZ delineated by laminin at 4 weeks post injury (Fig.3). We find that BDA labeled axons were found much more in DREZ in ISP group (Fig.4). For the functions recovery testing, animals in ISP group showed a significant refunction in peripheral afferent stimulation (Fig.5), in sensory-motor behavioral tests (Fig.6), and electrophysiology test (Fig.7) respectively. In this study, we demonstrated that systemic administrated ISP showed promising effects in morphology and function tests after dorsal roots injury. So, in the subsequent study in this project, we will involve in more in vivo and in vitro assays to verify the ISP regeneration promoted effects.

Biography :

Min Yao is a current Ph.D student in the department of surgery, the university of Hong Kong. Under the supervision of Professor Wutian Wu and Professor Gilberto Leung, she has obtained good experiences and techniques in molecular neurobiology and neuroscience. Within her period of study, she has achieved good progress in the pharmacological treatment after peripheral and central nervous system trauma. Her work has been accepted by some conferences and will be published soon.

E-mail: yaomin@hku.hk