Journal of Advanced Chemical Engineering

Synthesized polymers as multifunctional fluorescent sensors for bioanalysis

2^nd World Congress on Biopolymers

August 04-05, 2016 Manchester, UK

Yanqing Tian

South University of Science and Technology of China, China

Posters & Accepted Abstracts: J Adv Chem Eng

Abstract:

Optical sensors are important tools for cellular imaging and analysis, for probing cellular metabolism, for understanding pathways and biological and physiological processes, and for diagnosing diseases and cancers. We have used advanced polymer approach to synthesize several series of fluorescent optical sensors (pH, oxygen (O2), potassium ion (K+), and glucose sensors) suitable for intracellular and/or extracellular imaging and analysis. For example, we integrated individual sensors as dual sensors using polymeric synthesis techniques for simultaneous multi-parameter measurements. Of particular value for application in the complex biological environment, some of the sensors emit two or even three colors in response to the same excitation wavelength, enabling ratiometric measurements and providing superior measurement accuracy. For example, we have prepared a dual pH/O2 sensor with three emission colors. The tri-color sensor composed of a blue emitter as an internal build-in reference probe, a green emitter as the pH probe, and a red emitter as the oxygen sensor. The build-in reference probe is unresponsive to pH or O2. It was demonstrated that this polymerbased tri-color sensor is capable for measuring the changes of pH and oxygen concentrations in photosynthetic cyanobacteria possessing high autofluorescence. We also integrated glucose and oxygen sensor as ratiometric dual glucose/oxygen sensors for analysis of the glucose and oxygen consumption of bacterial cells and mammalian cells in real time. This dual glucose/oxygen sensor enables simultaneous measurement of glucose and oxygen, providing important information for continuous monitoring of glucose, diagnosing diabetes and hypoxia (low oxygen) related diseases and cancer, as well as the understating of biological processes of the metabolism.

Biography :

Email: Yanqing.Tian@asu.edu