Journal of Bioequivalence & Bioavailability

Synthesis of thiazole incorporated 4-oxo-thiazolindine derivatives endowed with broad spectrum antimicrobial potency

3^rd World Congress Bioavailability & Bioequivalence

March 26-28, 2012 Marriott Hotel & Convention Centre, Hyderabad, India

K. M. Rajpara and N. C. Desai

Posters: J Bioequiv Availab

Abstract:

A n isocratic RP-HP LC method was proposed for the determination of Olopatadine in pharmaceutical formulations. Olopatadine hydrochloride is an anti-allergic agent with histamineH1 receptor antagonistic action, prescribed for patients with signs and symptoms of AR, chronic urticaria, eczema dermatitis, prurigo, pruritis cutaneous, psoriasis vulgaris and erythema exsudativum multiform. It is chemically {(11Z)-11-[3-(dimethylamino) propylidene]-6, 11-dihydrodibenzo [b, e] oxepin-2- yl} acetic acid Olopatadine ophthalmic solution, which inhibits the pro-inflammatory activity of conjunctival mast cells, is an effective therapy for allergic conjunctivitis. Olopatadine inhibits the capsaicin-induced sneezing response. Isocratic elution was performed using water-acetonitrile mixture along with tri ethyl amine as mobile phase. The overall run time was 10 min. and the UV detection was carried at 246 nm. 20 μL of sample was injected into the HPLC system. In the present work chromatographic separation was achieved by using a C-18 (250mm ? 4.6mm i.d., 5 μm particle size) column of Shimadzu Model CBM-20A/20 Alite, equipped with SPD M20A prominence photodiode array detector, maintained at 25 ?C. Linearity was observed in the concentration range of 0.1?200 μg/mL (R 2 = 0.999) and the method was validated as per ICH guidelines. The RSD for intra-day and inter-day precision were found to be less than 2 %. The percentage recovery was in good agreement with the labeled amount in the pharmaceutical formulations and the method is simple, precise, accurate and ro bust for the determination of Olopatadine