Agrotechnology

Synthesis of resveratrol dimer catalyzed by grapevine peroxidases

13^th International Conference on Agriculture & Horticulture

September 10-12, 2018 | Zurich, Switzerland

Sung Chul Park, Cha Young Kim, Jang Won Pyun, Yu Jeong Jeong and Jae Cheol Jeong

Korea Research Institute of Bioscience and Biotechnology, South Korea

Posters & Accepted Abstracts: Agrotechnology

Abstract:

The ability of grapevine to activate defense mechanisms against some pathogens has been shown to be linked to the synthesis of stilbenes by the plant such as resveratrol and viniferins. ?-Viniferin and ?-viniferin are stilbenoid compounds and a resveratrol dehydrodimer. In the previous study, viniferin synthesis is occurred by human cyclooxygenase-1 and horse radish peroxidase via dimerization of resveratrol. However, major biosynthetic genes involved in ?-viniferin and ?-viniferin biosynthesis have not yet been identified in grapevine. In the previous study, we found that co-treatment of methyl jasmonate (MeJA) and solubilizing agent PX of grapevine suspension cells resulted in enhanced production of ?-viniferin and ?-viniferin in the culture media. To identify resveratrol dimerizing genes in grapevine, we isolated 7 putative grapevine peroxidase genes (APX1, GPX2, VvPrx1, -2, -3, -4, -5) which were specifically up-regulated in MeJA+PX treatment using transcriptome analysis. To examine the enzymatic activity of their genes for production of viniferins, the peroxidase genes under the control of CaMV 35S promoter were transiently expressed in tobacco leaves. Among them, overexpression of VvPrx1, VvPrx2 and VvPrx3 exhibited strong guaiacol peroxidase activities. Furthermore, HPLC analysis revealed that crude protein extracts from tobacco leaves overexpressing VvPrx1 and -2 catalyzed dimerization of resveratrol to ?-viniferin in vitro. Our results suggest that VvPrx1 and VvPrx2 might be involved in resveratrol dimerization in grapevine cells.

Biography :

Sung-Chul Park received his Master’s Degree in Plant System Biology from Chungnam National University and has conducted graduate work on metabolic engineering. He is a PhD student at Korea Research Institute of Bioscience and Biotechnology (KRIBB). His project is finding molecular mechanisms of stilbene compounds dimerization in grape callus. He is interested in mechanisms of metabolite biosynthesis in plants.

E-mail: kimcy@kribb.re.kr