Journal of Food Processing & Technology

Synthesis of caprylins through the esterification of glycerol with caprylic acid by using immobilized lipase Influence of stable and abusive temperatures

Joint Conference on 8^th World Congress on Agriculture and Horticulture and 16^th Euro Global Summit on Food and Beverages

March 02-04, 2017 Amsterdam, Netherlands

Michele Vitolo, Juliana N R Ract and Sara A B Guebara

University of São Paulo, Brazil

Scientific Tracks Abstracts: J Food Process Technol

Abstract:

Statement of Problem: Caprylins are short chain acylglycerols having emulsifying and antimicrobial properties. They are nonionic surfactants that can be synthesized using lipase as biocatalyst resulting more pure and not degraded lipids. This study aimed to caprylins synthesis by esterification of caprylic acid and glycerol using immobilized Rhizomucor miehei lipase (Lipozyme RM IM®) as biocatalyst. Methodology & Theoretical Orientation: The effects of reaction conditions, i.e., temperature (30ºC-70ºC) and molar ratio caprylic acid/glycerol (2:1-6:1) were analyzed using a factorial design consisting of two factors, two levels and three central points. Response surface methodology was applied in order to achieve the best combination of temperature and molar ratio. The reaction time was set at 6h and synthesis occurred in a conventional batch reactor under reduced pressure, agitation of 250 rpm and temperature control. The reaction yield was calculated from the amount of free fatty acids by titration method. Caprylins present in the mixture after the reaction were identified and quantified by gas chromatography (flame ion detector; CP Sil 5 CB column (length=10 m and diameter=0.25 mm; filled with phenyl-methylpolysiloxane); flow gas (1 mL/min: hydrogen). Findings: The highest reaction yield achieved was 93%, when the molar ratio caprylic acid/glycerol and temperature were 2:1 and 50ºC, respectively. However, the lower yield (6.3%) occurred at molar ratio caprylic acid/glycerol and temperature of 2.58:1 and 64ºC, respectively. Conclusion & Significance: Analysis of variance (evaluated through ANOVA statistical method) showed that the applied model was statistically significant, insofar as the observed values have been located very near of the predicted line (Figure1). So, the parameters chosen (substrates molar ratio and temperature) affected strongly the synthesis of caprylins.

Biography :

Michele Vitolo has graduated in Pharmacy and has done his PhD in Biochemistry from the University of São Paulo (USP), Brazil. He is a Full Professor at USP’s Pharmacy School and Senior Researcher of CNPq. His research interest focuses on enzyme catalysis in membrane reactors.

Email: michenzi@usp.br