Journal of Nanomedicine & Nanotechnology

Synthesis and characterization of ligand anchored poly propyleneiminedendrimers for the treatment of brain glioma

7^th International Conference on Nanomedicine And Nanotechnology

June 28, 2023 | Webinar

Ashish Parashar and Vandana Sethi

Lloyd Institute of Management & Technology, India

Scientific Tracks Abstracts: J Nanomed Nanotechnol

Abstract:

The goal of this study was to develop and characterize Angiopep-2 attached PEGylated Polypropylene imine (PPI) dendrimers for Temozolomide administration to brain gliomas. For surface neutralisation, synthesized PPI dendrimers were conjugated with PEG-2000. PEGylated PPI dendrimers were further coupled with Angiopep-2 (ANG-PEG-PPP) for enhanced medication transport across the blood-brain barrier (BBB) into the area of the brain tumour. Temozolomide loaded ligand-conjugated PPI dendrimers (TAPP) were characterized for size, % drug loading, cumulative drug release, and cell line studies. The drug loading was found to be 57.42±0.8%. In vitro release profile exhibited an early burst release followed by zero-order kinetics (46.8±0.8% in 24 hours). TAPP dendrimers showed improved antiproliferative efficacy against C6 glioma cells in MTT assays and cellular uptake investigations. The ability of TAPP to target cancer cells was examined using an in vitro co-culture model of BCECs and C6 glioma cells. Our findings suggest that Angiopep-2 coupled PEGylated PPI dendrimers could be a suitable nanocarrier for Temozolomide delivery to brain gliomas. Keywords: Dendrimer; PEGylation, Temozolomide; Angiopep-2, Glioblastoma Multiforme. Recent publications: 1. Parashar Ashish Kumar, Singh Gurdeep, 2021. Synthesis and characterization of temozolomide loaded theranostic quantum dots for the treatment of brain glioma.Jou. of Med. P’ceutical & Allied Sci. V 10-I 3, 1073, P-2778 – 2783. 2. Parashar Ashish Kumar, Singh Gurdeep, 2021. Synthesis and characterization of ligand anchored poly propyl eneimine dendrimers for the treatment of brain glioma. Jou. of Med. P’ceutical & Allied Sci. V 10-I 3, 1084, , P-2784 – 2789. 3. Parashar AK, Kurmi B, Patel P. Preparation and characterization of ligand anchored polymeric nanoparticles for the treatment of epilepsy. Pharmaspire 2021;13(1):1-5. 4. Pankaj Kumar Pandey and Ashish Kumar Parashar. Formulation, Characterization and Evaluation of Liposomal Hydrogel for the Treatment of Antibiotic Resistant Propionibacterium Acne. Current Research in Pharmaceutical Sciences 2021; 11 (02): 65 71. 5. Ashish K. Parashar, Preeti Patel, Arun K. Gupta, Balak Das Kurmi. Synthesis, Characterization and in vivo Evaluation of PEGylated PPI Dendrimer for Safe and Prolonged Delivery of Insulin. Drug Delivery Letters. 2019; 9: 3, 248 - 263.

Biography :

Ashish Parashar is dynamic personality with 14 years of experience in Academics and Research in pharmacy profession. He has completed his Ph. D degree from Oriental University Indore & M. Pharm. in Pharmaceutics from Dr. Harisingh Gour University, Sagar, M.P. Dr. Ashish has active participation in paper presentation at seminars and paper publication in National & International Journals. He has carried out various research project Grants and won several national awards such as JRF (AICTE) Award in 2006, best faculty of the year, in 2012 at Rishiraj College of Pharmacy, Indore & best faculty of the year in 2020 at Lakshmi Narayan College of Pharmacy, Indore. His main areas of interest are pharmaceutical technology, novel drug delivery systems, and pharmaceutics and drug delivery.