Brain Disorders & Therapy

Studying of the anti-neoplastic effect of the extracts of higher fungi in short-term cellular cultures of glial cells

International Conference on Neuro Oncology and Rehabilitation

July 21-22, 2016 Brisbane, Australia

O Makarenko, O Glavatskiy, I Vasilieva and I Shuba

Taras Shevchenko National University of Kyiv, Ukraine
Institute of Neurosurgery named after acad. A.P. Romodanov of AMS, Ukraine

Posters & Accepted Abstracts: Brain Disord Ther

Abstract:

Aim: The objective of our work was to investigate the influence on the cultures of glial tumors of the extracts of fungi Cordyseps sinensis and Ganoderma lucidum. Methods: For the analysis of glioma sensitivity to the medicines we used a method of short-term cultivation (72 hours) with the subsequent 0.4% trypan blue staining for assessment of both the total of cells in the sample and determination of the percentage of viable cells in control and experimental samples. Tissue fragments were processed, removing vessels and necrotized parts from them. The obtained suspension of cells was cultivated Dulbecco�??s Modified Eagle�??s medium (DMEM) with addition of 10% fetal bovine serum (FBS) (�??Sigma�?�, the USA)) in Petri dishes in the CO2 incubator in standard conditions. The samples containing not less than 70% of viable cells was included in the experiment. The concentration of medicines was counted taking into account the therapeutic dose and was added to the nutrient medium (experiment). Cell sensitivity to the action of medicines was estimated, comparing the amount of living cells in the experiment concerning control. Result: As a result of our researches, there was some statistically significant reduction of the percentage of living cells after cultivation with the extracts, concerning control in the culture of cells, obtained from the fragments of gliomas of the III-rd anaplasia degree. Conclusion: The results of our researches testify about the multidirectional influence of the preparations of fungi extracts under gliomas of various degree of anaplasia in the conditions in vitro.

Biography :

Email: makarenko.alexander.1954@gmail.com