Pharmaceutica Analytica Acta

Study of the carotenoid production kinetics by oxidative stress in Sporobolomyces ruberrimus and pigments extraction techniques

International Conference and Exhibition on Advances in HPLC & Chromatography Techniques

March 14-15, 2016 London, UK

Clarissa Detomi de Albuquerque, Ligia Alves da Costa, Jose Vladimir de Oliveira Cardoso and Jorge Luiz Ninow

Federal University of Santa Catarina, Brazil
University Positivo, Brazil

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Carotenoids are natural pigments produced by a wide variety of bacteria, yeast, filamentous fungi, plants, and algae. These pigments are natural antioxidants and represent a group of valuable molecules for applications in the pharmaceutical, chemical, food and feeding industries. Carotenoids are synthesized by Sporobolomyces ruberrimus in the intracellular environment and cell wall rigidity limits the extractability of such compounds, making the application of methods for recovery of these pigments necessary. The objective of this study was to study the carotenoid production kinetics in Sporobolomyces ruberrimus, seeking to enhance torularhodin biosynthesis and investigate pigment extraction methods (mechanical, chemical and high pressure). Three concentrations of dissolved oxygen (20%, 50%, and 80%) were used in submerged batch cultivation in bioreactor. The cell morphology, after the application of these methods, was verified by scanning electron microscopy. Besides it, after 72 h of fermentation, the biomass was concentrated and lyophilized for the application of supercritical extraction, a recent technique applied for the extraction of pigments. HPLC analyses were used for carotenoids identification and quantification. Results showed that the best condition for torularhodin biosynthesis (11.95 mg.L-1) was 50% of dissolved oxygen, with Xmax 13.13 g.L-1, �¼max of 0.12 h-1, a maximum production of carotenoids total of 30.61 g.L-1 and productivity of metabolites of 0.42 mg.L-1.h-1. The use of supercritical extraction using lyophilized cells pretreated by soaking with liquid nitrogen and the use of ethanol as co-solvent proved to be the best treatment in terms of yield (3.11%) and extracted carotenoids (28.99 �¼g.g-1 of dry cell).

Biography :

Email: ligiacardoso@up.edu.br