Agrotechnology

Studies of genetic diversity analysis of soybean (Glycine max L.) by PCR based molecular markers

3^rd International Conference on Agriculture & Horticulture

October 27-29, 2014 Hyderabad International Convention Centre, India

Hirakant Kalpande, A W More, S K Chavan, T Anjaneyulu, V S PATIL and A N Naiknaware

Posters: Agrotechnol

Abstract:

Genetic diversity studies of 10 soybean cultivars of Glycine max L. was carried out using fifty RAPD primers of OPA, OPAC, OPC, OPB, OPG, OPL, OPH, OPR, OPX series. Twelve selected polymorphic RAPD marker produced 90 fragments of which 64 fragments were found to be polymorphic, resulted in 72.74 percent polymorphism. All cultivars could be distinguished based on RAPD profiles unweighted pair group method with the airthmetric average (UPGMA) was used to construct dendrogram. The value of similarity coefficient of Dendrogram calculated by RAPD marker ranged from 0.313 to 0.618. In addition cluster analysis (CA) was used in order to determine genetic variation among soybean cultivars. The results obtained in this study showed narrow genetic base among the cultivars and can be used in selecting divergent parents for breeding and mapping purposes.