Journal of Nanomedicine & Nanotechnology

Structural and molecular characterization of biochemical interactions thorough microscopic examinations

22^nd International Conference and Expo on Nanoscience and Molecular Nanotechnology

November 06-08, 2017 | Frankfurt, Germany

Maryam Kamalipourazad, Mohammad Amir Parvin and Hassan Nezammahalleh

Tarbiat Modares University, Tehran, Iran
Science and Research Branch, Islamic Azad University, Bushehr, Iran
Young Researchers and Elite Club, Islamic Azad University, Tehran, Iran

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Biochemical interactions result in various nanostructures in both intra and extracellular environments. Design of nanostructures in vitro and study of biochemical assemblies in vivo need an understanding of their formation mechanisms and their structural characteristics. Diphenylalanine self-assembly in a synthetic media and lipid aggregation inside algal cells are two examples of the biochemical interactions. Microscopic techniques give us the capability to predict the behavior of these self-assembled nanostructures and to identify their characteristics in different media. The kinetics of self-assembly can be well investigated by online monitoring the organization phenomena. The dissolution process of a given nanostructure can also be monitored by observing the solvation phenomena so as to find the relevant factors which would influence the dissolution process. For example, different solvents would dissolve nanostructures in a chain type reaction or in a power law nucleation with consequent interface advance. Dissolution of diphenylalanine nanostructures in water and in methanol are instances of these reaction types, respectively, Figure 1. The size and shape characteristics of the nanostructures are strongly influenced by the surrounding environment. Electron microscopy techniques provide us with the information on crystal habit modification. For example, the morphological evolution of diphenylalanine nanostructures makes possible the identification of ion types in the solution of the dipeptide. In addition, staining of assembled biochemicals and their cellular localization are of the other activities carried out in our investigations. Monitoring the aggregation of lipids and their location in living cells using fluorescent dyes e.g., Nile red, can be mentioned as an example, Figure 2. This would help us to implement correct action for cell treatment.

Biography :

Maryam Kamalipourazad has completed her PhD at the age of 30 years from Tarbiat Modares University. She has published more than 5 papers in international journals. She experiences in Medicinal plants and Natural products, Plant Cell Cultures, Biochemistry (HPLC, Enzyme activity measurement, protein electrophoresis,..), Plant physiology and Plant Environmental Stress Physiology (Anti-oxidant activities, measurement of Flavonoids, carbohydrates, amino acids, reactive oxygen species (ROS) contents), Molecular techniques: Extraction DNA and RNA from plant, bacteria, mitochondria and chloroplast, cDNA synthesis, primer designing, identification of gene expression levels by qRT-PCR analysis, extraction of plasmid, cloning, transformation (transgenic hairy root and callus), Green Nano.