Health Care : Current Reviews

Single nucleotide polymorphisms and the risk of venous thrombosis in Latvia

4^th Global Summit on Healthcare

November 09-10, 2015 Dubai, UAE

Irina Kajuna, Vita Rovite1, Liene Nikitina-Zake1, Janis Klovins1, Helena Mikazane2 and Valda Stanevica2

1Latvian Biomedical Research and Study Centre, Latvia 2Riga Stradins University, Latvia

Posters-Accepted Abstracts: Health Care: Current Reviews

Abstract:

Introduction: A number of single nucleotide polymorphisms (SNP) have been linked to higher risk of venous thrombosis. The clinical value of SNP genotyping has not been established in patients with deep vein thrombosis (DVT). Well known genetic risk factors F5 rs6025 (Factor V Leiden) mutation or deficiencies in coagulation inhibitors are present only in about 30% of DVT cases. Objective: To investigate DVT risk associated with SNPs F5 (rs6025), F2 (rs1799963), SELE (rs5361), SERPINC1 (rs2227589), FGG (rs20066865), CYP4V2 (rs13146272), F11 (rs2289252), GP6 (rs1613662) genes. Materials & Methods: The data collected in 2013 year for 52 patients with confirmed DVT and average age 59.9±15.2. In the control group were included 235 (110 men and 125 women) individual average age 53.1±14.1, body mass index (BMI) 28.12±4.96. Genotyping was performed using an Applied Bio-systems Taq-Man SNP Genotyping Assay after manufacturerâ??s protocol on ViiAâ?¢ 7 Real-Time PCR System. Statistical analysis was carried out with Plink 1.06 software. The additive model of inheritance was used in logistic regression for each SNP adjusting for sex, age, BMI. Results: From DVT patients were 32 women (62%) and 20 men (38%), BMI 32.4±26.2. The F5 (rs6025) gene polymorphism was significantly associated with higher DVT risk p=0.017, OR=4.37. Polymorphisms in genes SERPINC1 (rs2227589), FGG (rs20066865), F11 (rs2289252) showed significant association with deep vein thrombosis - SERPINC1 (rs2227589) p=0.05, OR=0.41; FGG (rs20066865) p=0.018, OR=1.79; F11 (rs2289252) p=0.028, OR= 1.65 but due to small group this association did not withstand the permutation test and Bonfferoni correction. Polymorphisms in genes F2 (rs1799963), SELE (rs5361), CYP4V2 (rs13146272), GP6 (rs1613662) did not show significant association with DVT accordingly F2 (rs1799963) p=0.62; SELE (rs5361) p=0.71; CYP4V2 (rs13146272) p=0.81, GP6 (rs1613662) p=0.82. Conclusions: 1) For patients with DVT statistically relevant connection was found with F5 (rs6025) gene polymorphism. 2) Polymorphisms in genes SERPINC1 (rs2227589), FGG (rs20066865), F11 (rs2289252) showed significant association with deep vein thrombosis, but larger group is required to prove this association.

Biography :

Irina Kajuna has completed her MD at the age of 24 years from Riga StradiÅ?š University and postdoctoral studies from Riga Stradinš University. She is the doctoral student at Riga StradiÅ?š University from 2013 year. She is a teacher at Riga StradiÅ?š University from 2014 year.

Email: irina.kajuna@gmail.com