Pharmaceutica Analytica Acta

Simultaneous determination of plant growth regulator naphthalene acetic acid and naphthalene acetamide in orange, banana peels, soil and lake water extracted through modified and miniaturized QuEChERS method and ion chro

International Conference and Exhibition on Advances in HPLC & Chromatography Techniques

March 14-15, 2016 London, UK

Muhammad Nadeem

Zhejiang University, China

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

A new isocratic simple, selective and highly sensitive with direct injection of sample has been developed for the simultaneous separation determination of two plant growth regulators (PGR) extracted by modified and miniaturized QuEChERS method from orange, banana peels soil and lake water using ion chromatography coupled with fluorescence and UV detector. The proposed analytical and new modified and miniaturized extraction method spiked with 5 μg/L, 15 μg/L and 25 μg/L exhibit satisfactory recoveries 85-111% in samples with relative standard deviations of less than 8%. After having been optimized, this new fluorescence method was validated for linearity (R2>0.98.8), Limit of detection (9 ng/kg-35 ng/kg), precision (%RSD<5.05), accuracy and matrix effect. The matrix effect was evaluated and compensated with matrix matched calibration. The applicability of the developed modified and miniaturized QuEChERS ion chromatography FLD/UV method was demonstrated by determining the presence of NAD and NAA in orange, banana peels and soil and lake water.

Biography :

Email: nadeemishaq@yahoo.com nadeem@zju.edu.cn