Journal of Food Processing & Technology

Simultaneous detection for multiplexed mycotoxins by using immunological and LC-MS/MS in food and feed

2^nd International Conference on Food Safety and Regulatory Measures

June 06-08, 2016 London,UK

Zhaowei Zhang and Peiwu Li

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, P.R.China
Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, 430062, P.R.China
Key Laboratory of Detection for

Scientific Tracks Abstracts: J Food Process Technol

Abstract:

Multiplexed mycotoxins with strong carcinogenesis and toxicity are fatal threats in food and feed safety, and require highly sensitive and high-throughput detections greatly. Rapid immunoassay and arbitration detection methods play a critical role on two sides of one coin. In the rapid immunoassay, a series of high specific and high affinity monoclonal antibody, recombinant antibody, and nanobody against aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEA), etc., were developed as the key recognition reagents. Based on these specific antibodies, simultaneous detection for multiplexed mycotoxins was studied by using the Au (or Europium)-based lateral flow strip and non-fouling antigen microarray. The limit of detection was lowered down to pg mL-1 level (0.3 pg mL-1), depending on mycotoxins in food and feed samples. On the other hand, simultaneous arbitration detection method based on LC-MS/MS was investigated. Either multiplexed immunoaffinity column or solid phase extraction column was used in the sample extraction. The internal standard allowed precise determination of mycotoxins regardless of matrices. Multiplexed mycotoxins (AFB1, B2, G1, G2, OTA, ZEA, and T-2 toxin) were successfully identified by using a new multi-immunoaffinity column in a single run. Furthermore, a promising proposal was suggested to achieve the rapid, sensitive, ultra high-throughput detection of 96-384 contaminants in food and feed, including biotoxins, pesticides, veterinary drugs, etc., based on immunochemiluminescence biosensors using Hadamard transformation imaging (iHT).

Biography :

Zhang Zhaowei is an analytical chemist in Oil Crops Research Institute. He received BSc/MS and PhD degrees from Wuhan University and Huazhong University of Science and Technology, China. He is currently a key stuff in the Key Laboratory of Detection for Biotoxins, MOA, PRChina. He published over 30 peerreviewed papers and 20 issued patents. His research area includes the development of biosensors (lab-on-a-chip) based on nanomaterials and the combined chromatography with mass spectrometry for chemical contaminants (as mycotoxin, pesticide, etc.) in the agro-food. He won the honorary title of Ten Thousand People Plan (Young Talent) in China.

Email: zwzhang@whu.edu.cn zhaowei_zhang@126.com