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Simple and versatile synthetic polydopamine based surface supports reprogramming of human somatic cells and long-term self-renewal of human pluripotent stem cells under defined conditions
7th Annual Conference on Stem Cell and Regenerative Medicine
August 04-05, 2016 Manchester, UK
Ping Zhou
Peking University, China
Scientific Tracks Abstracts: J Stem Cell Res Ther
Abstract:
Human pluripotent stem cells (hPSCs) possess great value in the aspect of cellular therapies due to its self-renewal and potential to differentiate into all somatic cell types. A few defined synthetic surfaces such as polymers and adhesive biological materials conjugated substrata were established for the self-renewal of hPSCs. However, none of them was effective in the generation of human induced pluripotent stem cells (hiPSCs) and long-term maintenance of multiple hPSCs, and most of them required complicated manufacturing processes. Polydopamine (PDA) has good biocompatiblity, is able to form a stable film on nearly all solid substrates surface, and can immobilize adhesive biomolecules. In our study, carboxymethyl chitosan was used as a linker to orthogonally and controllably attach adhensive peptide to PDA coated cell culture plates for the culture of hPSCs. This synthetic surface was demonstrated that, it not only support the reprogramming of human somatic cells into hiPSCs under defined conditions, but also sustain the growth of hiPSCs on diverse substrates. Moreover, the proliferation and pluripotency of hPSCs cultured on the surface were comparable to Matrigel for more than 20 passages. Besides, hPSCs were able to differentiate to cardiomyocytes and neural cells on the surface. This polydopamine-based synthetic surface represents a chemically defined surface extensively applicable both for fundamental research and cell therapies of hPSCs.
Biography :
Ping Zhou is currently pursuing his PhD from Peking University, China. He has published 5 papers in reputed journals.
Email: zhp07@pku.edu.cn