Agrotechnology

SDS-PAGE analysis for seed storage proteins in drought tolerant rice genotypes

2^nd International Conference on Agricultural & Horticultural Sciences

Radisson Blu Plaza Hotel, Hyderabad, India February 03-05, 2014

Shafina Haque, S K Pradhan and O N Singh

Posters: Agrotechnol

Abstract:

Seed storage protein analysis represents a valid improved approach for varietal identification and genetic purity analysis. It is a useful tool for studying genetic diversity via sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) in a short period of time. This study was aimed at exploiting the genetic variations with respect to seed storage proteins among 24 reproductive stage drought donor rice lines along with the susceptible checks through SDS-PAGE analysis. The experiment was carried out in the rice biotechnology laboratory, Crop Improvement Division, CRRI, Cuttack during 2013 following Laemmli, 1970 procedure. The concentration of the extracted protein samples was determined using Bradford assay (1976) using different concentrations of the samples against the control. Gel photographing and documentation were carried out using Syngene gel documentation system. The present investigation revealed that the drought tolerant rice genotypes differed from each other with respect to the total number of protein bands in each of the two zones. A maximum of 12 bands were observed in germplasm line Jhona 349 and the lowest number of four bands were obtained in case Kalakeri and T136. Twelve genotypes namely, Black Gora (NCS12), Sambha Mahsuri, IR 20, Sarjoo 50, Tam Cau 9A, Koshihikari, Saita, Kalakeri, BR 21, Nan Te Hao, Dhalasaita, T 136 did not exhibit any band in this zone. The homology of genotypes (per cent similarity) for protein bands varied from 0 to 94% among different varieties. In other words, per cent of heterogenity percentage between all possible pairs of the genotypes varied from 94% to 0%, which provided adequate genetic basis to study the variability for storage proteins among the studied genotypes. The data revealed that genotype Naveen with CR 143 2-2 showed highest homology of 94%. On the other hand genotype Basmati 370 exhibited maximum heterogeneity with Mahulata.

Biography :

Shafina Haque has completed M.Sc. in Genetics and Plant Breeding from Calcutta University in 2010 and presently working as Senior Research fellow as well as Ph.D. scholar under the guidance of Dr. O. N. Singh and Co- guidance of Dr. S. K. Pradhan at Central Rice Research Institute, Cuttack, Orissa.