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Screening of novel marine actinomycetes Streptomyces Fragilis g12 for production of protease enzyme
International Conference and Exhibition on Food Processing & Technology
November 22-24, 2012 Hyderabad International Convention Centre, India
Chakri S, Ch.V. Satya, Sridhar Chowdary M., and Sri Rami Reddy D.
Posters: J Food Process Technol
Abstract:
Decrease in the rate of discovery of new compounds from terrestrial actinomycetes with an increase in the rate of reisolation of know compounds and the rise of antibiotic resistance warrants a search for actinomycetes producing novel bioactive compounds in underexplored area and are potential targets for the search of novel actinomycetes. In the present study actinomycetes were isolated from marine sediments collected off the coast of Visakhapatnam and samples collected from Coringa mangrove forest, Kakinada, Andhra Pradesh. An isolate G12 from Coringa mangrove forest showed antibacterial activity against Escherichia coli, Bacillus licheniformis and Rhizobium radiobacter. In addition, the isolate G12 also exhibited protease producing capability. Fermentation condition viz., incubation time incubation temperature inoculum age, initial pH, carbon and nitrogen sources were optimized for maximizing the antibacterial metabolite and protease production. Physiological and biochemical characteristics of the isolate were studied by the conventional method and identified Streptomyces fragilis G12 by the Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh. The optimized conditions for protease production were; incubation time-7 days; Incubation Temperature- 320C; inoculum age- 6 days; initial pH- 8.0; carbon source- Maltose; nitrogen source- beef extract. . With the optimized conditions employed the protease production was 60.7U/ mL.This study shows that the isolated mangrove actinomycetes Streptomyces fragilis G12 has strong antibacterial potential along with moderate protease activity and is a potential candidate for industrial use.