Journal of Bacteriology & Parasitology

Screening and characterization of drug targets in Pseudomonas aeruginosa: Prediction of the homology model, dynamics, and docking towards drug designing

International Congress on Bacteriology & Infectious Diseases

November 20-22, 2013 DoubleTree by Hilton Baltimore-BWI Airport, MD, USA

Madhusudana P, Chaitanya M, Babajan B, Shobha rani, Anuradha C. M, Chitta Suresh Kumar and K. R. S Sambasiva Rao

Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Pseudomonas aeruginosa (PA01) is one of the most important and studied opportunistic Gram negative bacterial strains, which have a great potential to infect human beings as well as other mammals. In the present work unique enzymes were identified from PA01 by comparative metabolic pathway analysis. We have identified nine enzymes that are unique to the pathogen PA01 and are not similar with host H. sapiens and can be considered for rational drug design. 437 distinct potential drug targets were also identified from different metabolic pathways; as a case study, one of the unique enzymes, formate dehydrogenase gamma sub-unit (FDHH) is selected for prediction of 3D structure by MODELLER9V9, energy minimized by PROCHECK, WHATIF, and PROSA-2003. The predicted model was docked with substrate & their analogues; Complex Dynamics were performed for to identify more flexible and rigid residues of FDHH. The study was successful in listing out potential drug targets from the PA01 proteome.