Agrotechnology

Role of VPNB1 in vascular tissue differentiation

16^th International Conference on Agriculture & Horticulture

August 16-17, 2021 WEBINAR

Varvara Podia, Maria Theodorou, Dimitra Milioni and Kosmas Haralampidis

National and Kapodistrian University of Athens, Greece
Agricultural University of Athens, Greece

Scientific Tracks Abstracts: Agrotechnology

Abstract:

VPNB1 is a low weight armadillo repeat, nuclear and cytoplasmic protein exclusively expressed in the vascular tissue and appears to be involved in xylem and phloem differentiation. Data from NCBI demonstrate the presence of 4 armadillo motifs in the N-terminus end of the VPNB1 protein and indicate this region as the putative peptide binding site, while the C-terminus end is free of protein motifs. VPNB1 shows a dual localization, since N-terminus fusion with YFP guide the VPNB1 protein to nuclear bodies, and N- or C-terminus fusion with GFP direct the protein to the cytoplasm. Interestingly, VPNB1 is present in the gene expression profile of every global microarray analysis associated with the vascular tissue differentiation. During the implementation of a Vascular Cell Induction Culture System Using Arabidopsis Leaves (VISUAL) in wild type Columbia and vpnb1 cotelydons we observed less inducted cells in vpnb1 than in wild type cotyledons. Furthermore, under these inductive conditions, vpnb1 cotyledons display and increased frequency of vasculature interruptions compared to wild type. The presence of SNBE (Secondary wall Nac Binding Elements) in the VPNB1 promoter sequence, the confirmed induction of VPNB1 expression by the SND1 and VND7 transcription factors (via SNBE), as well as the specific vascular tissue expression pattern and the data from the VISUAL GUS expression system, are in line with the significant role of VPNB1 during vascular tissue differentiation.

Biography :

Varvara Podia is affiliated to National and Kapodistrian University of Athens. She is a recipient of many awards and grants for his valuable contributions and discoveries in major area of Plant Molecular Biology, Plant Biotechnology, Plant Genetics etc. Her international experience includes various programs, contributions and participation in different countries for diverse fields of study. Her research interests reflect in his wide range of publications in various national and international journals.