Journal of Microbial & Biochemical Technology

Role of Ralstonia solanacearum extracellular lipopolysaccharides as molecular mimicry

International Conference and Summit on Industrial & Pharmaceutical Microbiology

October 17-18, 2016 Kuala Lumpur, Malaysia

Sharanniah Umesha

University of Mysore, India

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Ralstonia solanacearum is an economically important pathogen, because of its production of extracellular lipopolysachrides (EPS) at infection sites as a virulence factors. Humans are more sensitive to LPS than other animals it is responsible for septic shock. LPS acts as a physical barrier providing bacteria protection from its surroundings. Most of the Gram negative bacterial lipopolysachride receptors show similar homogeneity in both plants and animals. In present study, we elucidated the role of extracellular lipopolysachride (EPS) in R. solanacearum pathogenesis. SSCP and LSSP were developed to molecular characterize Ralstonia solanacearum DOB-R1 strain from suspicious bacterial wilt fields. Extracellular polysaccharide (EPS) were extracted from R. solanacearum strain DOB-R1, the partially purified EPS was air-dried. The morphological and structural characterization was assessed by SEM and AFM. Chemical characterization of the different EPSs was carried out using spectroscopic methods, purified through HPLC and mass of the compounds were determined by LC-MS. Complete structural elucidation through IR and NMR analysis. Seed quality parameters were increased and disease incidence was decreased upon EPS treatment. The antioxidant enzymes viz., APX and GPOD were rapidly increased in highly susceptible (HS) cultivars upon EPS treatment. The activities of these enzymes were confirmed by amplification of APX and GPOD defense genes. The developed molecular detection and elicitor which are the valuable tools and eco-friendly approach to manage plant diseases. The implications involved in handling human pathogens can be overcome with these plant pathogens. Thereby this study finds the applications in the industrial microbiology.

Biography :

Email: pmumesh@gmail.com