Journal of Stem Cell Research & Therapy

Role of beta tubulin in leukemia progression

3^rd International Conference and Exhibition on Cell & Gene Therapy

October 27-29, 2014 Embassy Suites Las Vegas, USA

Raza Faizan, Monteil-Cristina and Tahir Shamsi

Scientific Tracks Abstracts: J Stem Cell Res Ther

Abstract:

Microtubules regulation helps in the development and maintenance of cell shape, growth, division, motility and in intercellular signaling. As a result the unstable state of these microtubules can be crucial for proper functioning of cell including development of cancer cell. One of the well known leukemia type CML is also progressed by microtubules. Our aim is to study the expression of tubulin isoforms during the CML chronic and blast phases and also the influence of transcription factors, activated by BCR/ABL, on the expression of these beta tubulins. We found during our bioinformatics transcription factors cyclosporine A (CREB) is involved to inhibit the tubulin gene. We cultured the leukemia cell lines KU812 and TCC-S and after incubation we seed and treat the cell lines with (cyclosporine A) with different concentration and time of interval. Cell proliferation result revealed that after 24 and 48 hours, TCC-S cells incubation with 1 mM concentration of cyclosporine A inhibitor (CREB) inhibits TCC-S cell line proliferation effectively in blast phase while 10mM concentration of cyclosporine A inhibitor (CREB) is significant to inhibit KU812 cells in chronic phase of leukaemia. We have also done western blotting to identify the change in tubulin protein but it doesn?t give any result however it could be repeated for future work. Furthermore we assess the change in tubulin gene by using qPCR with different tubulin isoforms and we found that the relative expression of tubulins isoforms in tubulin gene is usually up and high regulated in chronic phase comparatively blast phase of leukemia. That suggests that TUBB could be used as biomarkers for leukemia pathology. It is also believe that in chronic phase of leukemia, there would be high ratio of these markers.

Biography :

Raza Faizan is currently holding a Master's degree from Nottingham Trent University (UK) in Applied Bio Science (Molecular Biology). He was awarded for Bachelor's degree in laboratory Science from DUHS-IMT Karachi in December 2009. His academic credentials are certified from World Education Services (WES) Canada. He worked as permanent employer in the molecular department of National Institute of Blood Disease from January 2010 - June 2012. His combine skills made him qualify to achieve MSc applied bioscience Scholarship from Nottingham Trent University, UK. More recent, he is working with NHS- Blood and Transplant in Immunogenetics Department to explore his technical skills on Molecular level. He further want to purse his education in molecular till post doctorate, if he might get scholarship's opportunity.