Journal of Bacteriology & Parasitology

Recent acquisition of CTX-M-15-encoding gene by Pseudomonas aeruginosa

International Congress on Bacteriology & Infectious Diseases

November 20-22, 2013 DoubleTree by Hilton Baltimore-BWI Airport, MD, USA

Ketrin Cristina da Silva, Marina Moreno, Givago Faria Ribeiro da Silva, Barbara Let?cia Pereira Costa, Vasco Tulio de Moura Gomes, Pedro Henrique Nogueira Lima Filsner, Carlos Emilio Cabrera Matajira and Andrea Micke Moreno

Posters: J Bacteriol Parasitol

Abstract:

The acquired Ambler class B metallo beta-lactamases (IMP-, VIM-, SPM-, GIM-types) and extended spectrum beta-lactamases (ESBL: GES-, VEB-, PER-types) are widely described among Pseudomonas aeruginosa isolates, an opportunistic pathogen that causes severe infections in hospital settings. However, CTX-M-15 has not been reported among non-fermentatives and blaCTX-M-2 is restricted to P. aeruginosa isolated from nosocomial infections. In a study conducted to evaluate the dissemination of resistant phenotypes in Enterobacteriaceae from Brazilian swine herds, were isolated three P. aeruginosa strains with reduced susceptibility to third-generation cephalosporins. One single isolate (190A) harbored blaCTX-M-15 and both (220B and 228A) strains produced CTX-M-2-type ESBL, which were grouped in two clusters according to ERIC-PCR fingerprinting. The 228A and 220B strains exhibited a minimal inhibition concentration (MIC) of 128 mg/L and 190A of 32 mg/L to ceftriaxone, a broad-spectrum cephalosporin currently used in Brazil to treat P. aeruginosa infections (Picao, 2009). Moreover, additional resistance to quinolones was confirmed. Here, for the best of our knowledge, it is described for the first time the acquisition of blaCTX-M-15 by P. aeruginosa and the establishment of blaCTX-M-2 in Brazilian swine herds, which is a major public health concern because the spread of these resistance genes may result in treatment failure, increasing morbidity and mortality, longer hospital stays, higher costs, and further carbapenems administration, which are often reserved as the drugs of last resort for multidrug resistant pathogens. These findings demonstrate the need of rational use of antimicrobials in livestock and implementation of measures to control dissemination of resistant pathogens through food-production chain.

Biography :

Ketrin Cristina da Silva has completed her M.Sc. at the age of 24 years at Sao Paulo University. Currently, she is a doctoral student supported by FAPESP- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, and investigates beta-lactamases production by gram-negatives isolated from food-producing animals in Brazil. She is a young researcher and has recently published her master results in a high impact journal in antimicrobial resistance area.