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Rapid detection of microcystin-LR in biological fluids by functionalized magnetic gold nanoparticles and surface enhanced Raman spectroscopy
9th Nano Congress for Next Generation
August 01-02, 2016 Manchester, UK
Waleed A Hassanain, Emad L Izake and Godwin A Ayoko
Queensland University of Technology, Australia
Posters & Accepted Abstracts: J Nanomed Nanotechnol
Abstract:
Rapid, selective, sensitive and cost effective label free SERS method has been developed for the selective isolation and determination of Microcystin-LR (MC-LR) toxin in biological sample. MC-LR antibody fragments were used as a recognition layer onto magnetic core gold nanoparticles to extract MC-LR from horse plasma matrix within 30 minutes. The purified MC-LR was quantitatively determined by surface enhanced Raman spectroscopy (SERS) and the results were cross validated by ELISA technique. The new SERS method showed high selectivity and sensitivity down to the femtomole detection limit. The new method has strong potential for the rapid screening of MC-LR in the field.
Biography :
Email: w.hassanain@qut.edu.au