Journal of Vaccines & Vaccination

Proteomic informed by transcriptomic towards candidate vaccine selection

5^th Asia Pacific Global Summit and Expo on Vaccines & Vaccination

July 27-29, 2015 Brisbane, Australia

Ana Domingos

Posters-Accepted Abstracts: J Vaccines Vaccin

Abstract:

The animal products consumption is expected to drastically increase in the next decades in parallel with their market price, and, huge losses in productivity may be expected due to animal diseases. Among these, tick-borne diseases remain a rising risk to animal as well to human health and control measures based on acaricides have shown severe limitations. Therefore, vaccines are an alternative option, by-passing acaricide resistance ticks, being environmentally friendly and economically rewarding. Current vaccines have shown restricted achievement due to deficient antigen selection which may be surpassed by ?omics? approach. Having as main target the selection and evaluation of new antigens aimed at vaccine trial, we performed RNA sequencing and compared the transcriptome and proteome of different Rhipicephalus spp. tacking in account parasite infection. After mass spectrometry, proteins were identified using the proteomic informed by transcriptomic method which combines deep sequencing transcriptomics and MS/MS allowing protein identification in the absence of a reference proteome. Proteomic results validated transcriptomic analysis and, the combination of these two approaches, provided strong support for the identification of relevant pathways in ticks. Based on these achievements we selected several genes for functional analysis and further cattle vaccination trials with recombinant antigens. The results showed both reduced tick infestation and parasite infection, strongly suggesting the involvement of selected proteins in vector-pathogen interactions, recommending its inclusion in a vaccine targeting both arthropods from diverse locations and multiple pathogens.