Hee Sung Kim, Bok-Il Jang, Dong Gyu Choi, Sung Hwoan Cho and Minsu Ha
Korea Maritime and Ocean University, Korea
Daehan Feed Co. Ltd, South Korea
Posters & Accepted Abstracts: J Aquac Res Development
The optimum protein requirement in the microdiet for larval rockfish (Sebastes schlegeli) was determined. The newly-hatched larvae started to fed on rotifers at one day and ended at six day, and Artemia at six day and ended at 10 day until the start of the microdiet feeding experiments at 10 day post hatching (DPH). Rotifers and Artemia nauplii were enriched with Selco presso (INVE) before supply. 4809 day DPH larvae were distributed into 15 indoor 70 L square plastic tanks (300 larvae per tank) for the feeding trial. Two sized microdiets (0.31-0.48, and 0.48-0.63 micrometer in diameter) were supplied as fish grew: at 10 and 27 DPH for the former, and 24 and 29 day DPH for the latter. Fish meal, soluble fish protein concentrate, krill meal and wheat gluten are the protein sources in the experimental diets. Alpha-starch and dextrin, and fish oil were used as carbohydrate and lipid sources, respectively in the experimental diets. Five different levels of crude protein diets ranging from 42 to 58% with 4% increment at the expense of dextrin at a constant energy level (4.42 kcal/g diet) were prepared in triplicate. The experimental diets were manufactured by Daehan Feed (Incheon, Korea). As dietary protein levels increased, all essential and non-essential amino acids contents increased. Survival of larval rockfish ranging from 54.3 to 55.2% was not significantly different between the granulated microdiets containing different levels of crude protein. Weight gain of larval rockfish fed with 54% protein diet was significantly higher than that of fish fed with all other diets, followed by the 58, 50, 46 and 42% protein diets. Protein requirement in the granulated microdiet was estimated to be 54.0% based on the broken-line analysis of weight gain of larval rockfish.
Hee Sung Kim is pursuing his PhD in Department of Convergence Study on the Ocean Science and Technology, Korea Maritime and Ocean University, Busan, Korea. His major is Aquafeeds Nutrition and Engineering.
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