Journal of Clinical & Experimental Pharmacology

Protective effect of coenzyme Q10 against acetaminophen-induced nephrotoxicity

36^th World Congress on Pharmacology

July 25, 2023 | Webinar

Mahmood Hassanshahi

Rafsanjan University of Medical Sciences, Iran

Posters & Accepted Abstracts: J Clin Exp Pharmacol

Abstract:

Acetaminophen overdose causes kidney injury via oxidative stress and apoptosis induction. Coenzyme Q10 (CoQ10) has several effects such as antioxidant, anti-inflammation and anti-apoptotic properties. The aim of this study was to determine the protective effect of CoQ10 on acetaminophen-induced kidney injury in rats. In this study, 40 male Wistar rats were randomly divided to five groups (n=8) including control, CoQ10 10 mg/ kg, acetaminophen, acetaminophen+CoQ10 5 mg/kg and acetaminophen+CoQ10 10 mg/kg. Animals initially received a single dose of acetaminophen (700 mg/kg), then CoQ10 treatment was given as two i.p. injections at 1 and 12 h following acetaminophen administration. Then, animal were sacrificed and Malondialdehyde (MDA) concentration, Glutathione Peroxidase (GPx) and Superoxide Dismutase (SOD) activity levels were measured in the kidney tissue. The western blotting analysis was used to determine the Bax, Bcl-2 and cleaved caspase-3 proteins expression level in the kidney tissue. CoQ10 (10 mg/kg) significantly increased the GPx and SOD activities and also reduced the MDA concentration in the acetaminophen+CoQ10 10 mg/kg group versus acetaminophen group (P<0.05). Also, CoQ10 significantly decreased the cleaved caspase-3, Bax proteins expression level in the acetaminophen+CoQ10 10 mg/kg group versus acetaminophen group (P<0.05). This study showed that CoQ10 can ameliorate the acetaminophen induced acute renal injury at least via suppressing oxidative stress and apoptosis parameters. Keywords: Acetaminophen, Oxidative stress, Apoptosis, CoQ10, Antioxidant, Protective effect.

Biography :

Mahmood Hassanshahi is affiliated to School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. He is a recipient of many awards and grants for his valuable contributions and discoveries in major area of Medical Practioner. His international experience includes various programs, contributions and participation in different countries for diverse fields of study.