Journal of Nanomedicine & Nanotechnology

Preparation of silver nanoparticles by using biological and chemical method and study of their antibacterial activity

Nanotechnology Congress & Expo

August 11-13, 2015 Frankfurt, Germany

Rupam Soliwal and Sanchita Dass Roy

Poster-Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Silver nanoparticles play one of the major roles in the nanotechnology industry and have a wide-spread application in the field of electronics, agricultural and medical domains. In this paper, chemical as well as biological methods for the synthesis of silver nanoparticles are mentioned. For the chemical synthesis, sodium dodecyl sulphate was used as a stabilizing agent and silver nitrate as a metal salt precursor. Hydrazine hydrate and citrate of sodium were used as reducing agents. Biosynthesis method is an eco-friendly and economical technique for the synthesis of silver nanoparticles. For biological synthesis, the leaf extracts of Murraya koenigii and Azadirachta indica were used as reducing agents. The prepared nanoparticles were characterised by UV-Visible spectroscopy. The absorption spectrum obtained in UV-Vis confirmed the formation of silver nanoparticles by different reducing agents. The antibacterial activity was investigated on two types of bacteria, Escherichia coli and Pseudomonas aeruginosa, which are mainly present in sewage water by using Kirby-Bauer disc diffusion technique. Zone of inhibition indicated the antibacterial effect of silver nanoparticles.