Journal of Stem Cell Research & Therapy

Preparation and in vitro evaluation of chitosan/apatite composite for bone regeneration applications

Joint Event on 12^th Annual Conference on Stem Cell and Regenerative Medicine & 4^th Annual Conference on Biomaterials

June 04-06, 2018 | Prague | Czech Republic

Miriela Tomas, Yaimara Solis, Carlos Peniche, Andy Hernandez  and Luis J Cruz

Laboratories AICA, Cuba
BIOMAT- University of Havana, Cuba
University of Havana, Cuba
Polytechnic University of Valencia, Spain
Leiden University Medical Center (LUMC), The Netherlands

Posters & Accepted Abstracts: J Stem Cell Res Ther

Abstract:

Contemporary bone repairing and regeneration techniques require biomaterials able to bond tightly to new bone, to permit bone growth and propagation. In this context, special attention has been given to chitosan/apatite composites (CHI/Ap) for bone regeneration because they have favorable properties of both components: bioactivity and osteoconductivity provided by apatite and degradation and flexibility supplied by chitosan. In this research CHI/Ap composites with different weight ratios (20/80; 50/50; 80/20) were obtained using a methodology for obtaining the inorganic apatite material in situ inside the chitosan matrix. The composites were characterized by Fourier Transform Infrared Spectrometer (FTIR) indicating the presence of the main functional groups of each component in the material. In vitro physiological stability and enzymatic degradation resulting materials were evaluated using solutions of phosphate buffered saline (PBS, pH=7.4). Composites with higher polymeric content showed the lowest physiological stability and the highest enzimatic degradation. The material bioactivity was demonstrated by deposition of a calcium phosphate layer with apatite morphology on the composite surface after immersion in simulated body fluid (SBF, pH=7.4). Preliminary cytotoxicity tests evidenced that the studied materials did not modify the natural proliferation of the hamster ovary cells (CHO-K1), demonstrating their cytocompatibility under physiological conditions. The results suggest that chitosan/apatite composites obtained are promising materials for bone regeneration applications.

Biography :

Miriela Tomas has completed her Bachelor's degree from the Faculty of Chemistry at the University of Havana. She has been working in the Research Department at Laboratories AICA. She is currently doing a research stay to complete her Master's degree at the Radiology Department of Leiden University Medical Center (LUMC) in The Netherlands. She has presented 8 comunications in International Scientific Congresses.

E-mail: miriela9005@gmail.com