Journal of Vaccines & Vaccination

Preclinical evaluation of metastatic breast cancer microparticulate vaccine

Joint Event on 32^nd International Conference on Vaccines and Immunization & 4^th Annual Summit on Infancy, Child Nutrition & Development

November 09-10, 2018 | Atlanta, USA

Rokon Uz Zaman, Nihal S Mulla and Martin J D Souza

Mercer University, USA
Drake University, USA

Scientific Tracks Abstracts: J Vaccines Vaccin

Abstract:

Introduction: Breast cancer is the most fatal form of cancer in females. Even with early diagnosis, 20% of women still develop metastases. Immunotherapy is being explored to provide a better treatment option, as current therapies against breast cancer are mostly invasive and pose numerous adverse effects. With this in mind, our purpose has been to formulate and evaluate a micro particulate therapeutic vaccine to provide a new line of therapy for metastatic breast cancer. Methods: Vaccine microparticles were prepared by encapsulating 4T1 tumor associated antigens in cellulose polymer using spray dryer technology. In vitro characterization of microparticles was conducted. Expression of surface co-stimulatory molecules on dendritic cells treated with vaccine microparticles and other controls were determined by flow cytometer. To check the efficacy of the vaccine, vaccine microparticles were administered using microneedles in Balb/c murine breast cancer model. Metastasis of 4T1 tumor cells to different organs was measured by the 6-thioguanine clonogenicity assay. Results: The yield of the microparticulate vaccine, following spray drying was 80±5% w/w. The particle size was 1-4 ?m. Zeta potential was -7±2 mV. There were significantly higher amount of nitric oxide released in the supernatant of cells exposed to vaccine microparticles compared to blank microparticles. CD40, MHC II and CD80, MHC I expression were significantly higher in the vaccine microparticles group compared to blank microparticles and vaccine suspension group. The immunized animals showed significantly lower tumor growth compared to the naïve animals. Significant increase in the CD4+ T cell population was observed. Most of the immunized animal groups were rescued from metastasis. Conclusion: The whole cell lysate vaccine microparticles formulated by spray drying can potentially be an effective treatment for patients with metastatic tumor. Translational Impact: This vaccine can futher be used for preclinical and clinical studies and can be potentially used for personalized treatment of metastatic breast cancer in humans.

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