Journal of Food Processing & Technology

Potential effects of refined deep-sea water (RDSW) on tumor growth

International Conference and Exhibition on Food Processing & Technology

November 22-24, 2012 Hyderabad International Convention Centre, India

Yoshihiro Hataguchi, Kouji Nagawa, Yoshinari Ikegami, Hiroaki Takeuchi and Tetsuro Sugiura

Posters: J Food Process Technol

Abstract:

Our aim is to evaluate whether high-mineral RDSW influences the biological behaviors of tumor cells in vitro and vivo. Materials and Methods: RDSW produced from deep-seawater off Muroto in Kochi Prefecture. The deep-seawater was desalinated and conditioned to give 5 magnesium:calcium ratios of 1:2 (A), 1:1 (B), 3:1 (C), 1:0 (D) and 0:1 (E) at hardness of 1000, expressed by 5 types of high-mineral RDSW. The RDSW and distilled waters were used in this study as test and control waters.MKN-45 was cultured in RPMI1640 medium prepared by each RDSW and control waters for 72 hours. The cell viability was examined by MTT assay and was compared among the 6 mediums. The animals (15 male nude mice in each 6 group) were allowed free access to test or control waters during in vivo experiment. After two weeks, MKN-45 was injected in the subcutaneous part of posterior cervical region. Two weeks later, the animals were sacrificed and tumors and sera were obtained. The tumor size was measured. All tumors were subjected to western blotting with sera from all animals to evaluate the immunoreactivity whose antibody profile was compared among 6 groups. Results: Cell viability in vitro was 62-79% in 5 RDSW compared to control with statistical significance. Tumor volume was 54-80% in 5 RDSW compared to control with a significant difference. Therefore, high-mineral RDSW potentially possesses a repressive effect on tumor growth. Furthermore, 44 molecules were gained as candidate determinants involved in this phenomenon.

Biography :

Yoshihiro Hataguchi graduated from Himeji Institute of Technology University (1995) and belongs to Technical development division of Ako kasei Co., Ltd. One of the company?s management policies is to devote to healthy human life by producing a variety of high quality and effective foods and liquids with original technology. Yoshihiro Hataguchi, a section chief, is working to research and develop new goods based on deep-seawater which possess functional effect in vivo.