Journal of Nanomedicine & Nanotechnology

Porous nanoparticles entrapped pipette tips for sensitive detection of biomolecules

Nanotechnology Congress & Expo

August 11-13, 2015 Frankfurt, Germany

Chang Lei and Chengzhong Yu

Poster-Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

In recent years, nanomaterials with ordered pores have been widely used for biomolecules detection due to the properties of tuneable pore size, high surface area and easily surface modification. Particularly, the combinations of nanomaterial with some advanced techniques, such as mass spectrometry, ensure the accurate analysis of biomolecules. However, the analysis of some low abundance biomolecules (e.g. insulin) in complex biological samples still remains a big challenge. To address this problem, a combopore approach utilizes materials with different pore sizes for sample pre-treatment has been developed for the sensitive detection of insulin. This advanced approach has been applied prior to mass spectrometer or enzyme linked immunosorbent assay (ELISA) to achieve better detection sensitivity in urine/serum. Furthermore, a novel combo-pipette-tips with different porous materials entrapped is developed as ?smart? device for convenient pre-treatment. The application of nanoparticle entrapped pipette tips for sample pre-treatment has the advantages of easy operation and less sample loss. As both combo-pore approach and nanoparticle entrapped tips can be special designed for different targets, which hold great potential for application in the sensitive detection of various biomolecules of commercial and clinical significance.