Journal of Food Processing & Technology

Polyphenol profiles of barley sprouts at different growth stages and investigation of their antioxidative effects

4^th International Conference and Exhibition on Food Processing & Technology

August 10-12, 2015 London, UK

Woo Duck Seo, Kyung Hye Seo, Mi-Ja Lee, Hyeon Jung Kang, Kwang-Sik Lee, Song-Min Oh and Sun Lim Kim

Posters-Accepted Abstracts: J Food Process Technol

Abstract:

This study was the first to investigate polyphenol profiles and antioxidant properties in BS at different growth stages and cultivar.
The isolated compounds were identified as 3-O-Feruloylquinic acid, Lutonarin, Saponarin, Isoorientin, Orientin, Isovitexin,
Isoscoparin-7-O-[6-sinapoyl]-glucoside,Isoscoparin-7-O-[6-feruloyl]-glucoside, Isovitexin-7-O-[6-sinapoyl]-glucoside and Isovitexin-
7-O-[6-feruloyl]-glucoside using UPLC-PDA-ESI/MS analysis. Among them, compound 2-6 had a good ability to reduce free
radicals in 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities with IC50 values of 20.7-74.9 μM and 2-azino-bis-(3-
ethylbenzothiazoline-6-sulphonicacid (ABTS) radical cation scavenging activities with IC50 values of 5.7-32.0 μM (Compared with
quercetin as positive control, IC50=26.0 and 23.6 μM, respectively). Furthermore, compound (2, 4 and 5) pre-treatment cells were
diminished Reactive Oxygen Species (ROS) and increased cell viability without cytotoxicity. We validate this by showing that saponarin
(SA) is as inhibitor of ROS. The richest cultivar for SA was found to be the Keunalbori cultivar. The most abundant SA in barley sprout
showed 62-75% of the total polyphenols content at every stage. These results are valuable to determine the optimal times of harvest to
obtain the highest yield of polyphenols.

Biography :

Woo Duck Seo has completed his PhD from Gyeong Sang National University and Postdoctoral studies from Korea Institute of Radiological & Medical Science. He has
published more than 35 papers in reputed journals and has been serving as an Editorial Board Member of repute.