Journal of Nanomedicine & Nanotechnology

Polyelectrolyte coated liposome delivery systems against Group A Streptococcus (GAS) infection

13^th International Conference and Exhibition on Nanomedicine and Pharmaceutical Nanotechnology

July 24-25, 2017 | Rome, Italy

Istvan Toth

The University of Queensland, Australia

Keynote: J Nanomed Nanotechnol

Abstract:

We have developed an oral vaccine delivery system to prevent infection by GAS by encapsulating lipid core peptide (LCP) antigens into the liposomes. We synthesised the LCP construct by attaching C-16 lipoamino acid to J-14 (B-cell epitope derived from GAS M-protein) and P25 (CD4+ T helper cell epitope) using a microwave assisted solid phase peptide synthesis method. Liposomes were formulated incorporating the LCP and optimized for charge and lipid content using a thin film formation method. Optimized liposomes were coated with oppositely charged polyelectrolytes (positively charged trimethyl chitosan (TMC) and negatively charged sodium alginate) in a layer-by-layer approach. Optimized formulations were investigated for their efficiency of uptake by intestinal immune cells and ability to induce mucosal IgA and systemic IgG responses.

Biography :

Istvan Toth PhD, DSc, is a Chemical Engineer and an internationally recognized expert in Drug Delivery. His major research interests are immunoadjuvants, carbohydrates, lipids, peptides, nucleosides and nucleotides. New developments in drug/vaccine delivery are clearly likely to have enormous economic impacts upon the pharmaceutical and biotechnology industries. He is a Fellow of the Royal Australian Chemical Institute (FRACI) the Queensland Academy of Science and Art (FQA) and the Hungarian Academy of Sciences.

Email: i.toth@uq.edu.au