Journal of Stem Cell Research & Therapy

Platelet-rich concentrates differentially release growth factors for bone regeneration

7^th Annual Conference on Stem Cell and Regenerative Medicine

August 04-05, 2016 Manchester, UK

Zhong-Shuang Liu, Ying Li and He Wu

Harbin Medical University, China

Posters & Accepted Abstracts: J Stem Cell Res Ther

Abstract:

In recent years, one of the hot topics in tissue engineering and regenerative medicine is the development of controlled release systems for bone regeneration. This study aimed for a biomimetic strategy that designed scaffolds resultant from the mixing of Platelet-rich fibrin (PRF) , a growth factor-enriched endogenous scaffold, and lyophilized Platelet-rich fibrin( L-PRF) with different ratio, tailored for different delivery rates of growth fators, seeking delivery systems of bioactive factors for tissue healing. To test this concept in vitro, the biological effects of autologous PRF on rabbit BMSCs was investigated, including cell proliferation, osteogenic differentiation potential, alkaline phosphatase (ALP) activity, and mineralization nodes. Compared with PRF and L-PRF, mixed PRF (PRF and L-PRF at weight ratios of 1:1, 1:3, 1:5) induced a significant and continuous stimulation of proliferation on rabbit BMSCs throughout the 7-day incubation period. Furthermore, the mixed PRF promoted the osteoblastic differentiation of BMSCs by increasing ALP activity and mineralization nodes formation during the testing period. To investigate the effects of PRF on bone regeneration, critical size cranial bone defects were created and covered with PRF, L-PRF and mixed PRF (at weight ratio of 1:1). The results were analyzed by micro-CT and histology. Six and twelve weeks after surgery, mixed PRF group achieved a more effective way to do bone reconstruction. With the confirmation from the micro-CT results, which showed bone-like density at the margins of the defects and a homogeneous radiolucent area in the central part of the defect. A far better result compared with the others tested within the groups.These findings indicated that mixed PRF allowed for sustained-release growth factors, which was an effective strategy to overcome the challenge and to enhance the function of biomimetic constructs for bone regeneration.

Biography :

Email: liuzshuang@126.com