Bioenergetics: Open Access

Plasmatic ceramides profile in adolescents and its association with hepatic steatosis independently of obesity

3^rd International Conference on Lipid Science and Technology

December 11-12, 2017 | Rome, Italy

Jorge Maldonado-Hernandez, Gabriela E Saldana Davila, Monica I Pina Aguero, Benjamin A Nunez Garcia and Mardia G Lopez Alarcon

IMSS, Mexico

Scientific Tracks Abstracts: Bioenergetics

Abstract:

Objective: To assess the association between plasma ceramides and hepatic steatosis (HS) in adolescents, independently of obesity. Material & Methods: Ninety-four adolescents from two previous studies conducted and published by our crew were included. Study subjects were stratified in three groups: normal weight (n=18), obesity (n=34) and obesity + HS (n=42). The presence of HS was defined when AST/ALT ratio was <1. Ceramides subspecies (C14:0, C16:0, C18:0, C24:0 and C24:1) were determined by LC/MS. Results: All ceramides correlated inversely with the AST/ALT ratio and directly with ALT levels in plasma; the strongest correlation was observed among C14:0 ceramide (r=-0.54 and r=0.41, respectively; P<0.001). Furthermore, significant correlations were observed between cholesterol and all ceramides except for C24:1 ceramide. Fasting insulin and HOMAIR correlated directly with ceramides C14:0, C18:0 and C24:1. For assessing HS, a cut-off points of 10.3 nmol/L for C14:0 Ceramide reported a sensitivity of 92.7% and a specificity of 73.5% when normal weight and obesity groups (n=52) were compared against obesity + HS group (n=42). Positive and negative predictive values were 77.5% and 90.2%, respectively. Conclusions: plasma ceramides are closely associated with hepatic steatosis in adolescents. C14:0 ceramide could be a novel biomarker of HS independently of obesity. Recent Publications: 1. Maldonado Hernández J, Saldaña Dávila G E, Piña Aguero M I, Núñez García B A and López Alarcón M G (2017) Association between plasmatic ceramides profile and AST/ALT ratio: C14: 0 ceramide as predictor of hepatic steatosis in adolescents independently of obesity. Canadian Journal of Gastroenterology and Hepatology 2017:3689375. 2. Maldonado-Hernández J, Martínez Basila A, Salas Fernández A, Navarro-Betancourt J R, Piña Aguero M I and Bernabe García M (2016) The 13C-glucose breath test for insulin resistance assessment in adolescents: comparison with fasting and post-glucose stimulus surrogate markers of insulin resistance. Journal of Clinical Research in Pediatric Endocrinology 8(4):419-424. 3. Bernabe Garcia M, López Alarcon M, Salgado Sosa A, Villegas Silva R, Maldonado Hernandez J, Rodriguez Cruz M and Gordillo Alvarez V (2016) Enteral Docosahexaenoic Acid Reduces Analgesic Administration in Neonates Undergoing Cardiovascular Surgery. Annals of Nutrition and Metabolism 69(2):150-160. 4. Bernabe-Garcia M, Lopez-Alarcon M, Villegas-Silva R, Mancilla-Ramirez J, Rodriguez-Cruz M, MaldonadoHernandez J and Lagunes-Salazar S (2016) Beneficial effects of enteral docosahexaenoic acid on the markers of inflammation and clinical outcomes of neonates undergoing cardiovascular surgery: an intervention study. Annals of Nutrition and Metabolism 69(1):15-23. 5. Salas Fernández A, Maldonado-Hernández J, Martínez Basila A, Martínez Razo G and Jasso-Saavedra F (2015) The 13C-glucose breath test is a valid non-invasive screening tool to identify metabolic syndrome in adolescents. Clinical Chemistry and Laboratory Medicine 53(1):133-138.

Biography :

Jorge Maldonado-Hernández has his expertise in the development of non-invasive methods and biological markers for the diagnosis of metabolic disorders as hyperhomocysteinemia, insulin resistance and non-alcoholic fatty liver disease. He has extensive experience in the management of analytical techniques such as isotope ratio mass spectrometry, gas chromatography and liquid-mass chromatography. Recently, he has been interested in the effect of dietary lipids on the biosynthesis of ceramides in human muscle tissue.