Brain Disorders & Therapy

Pioglitazone exerts anticonvulsant properties in febrile-induced seizure model via modulating the inflammation and attenuating apoptosis

6^th International Conference on Brain Disorders and Therapeutics

September 13-15, 2018 | Copenhagen, Denmark

Hussein Allawi Hussein

Ferdowsi University of Mashhad, Iran

Posters & Accepted Abstracts: Brain Disord Ther

Abstract:

Seizures provoked by fever are common, and teach us about mechanisms of seizure generation early in life. So, animal models offer the hope of providing the mechanisms for simple and prolonged febrile seizures as well as enabling an understanding of pro-epileptogenic consequences of prolonged febrile seizures which eventually may promote temporal lobe epilepsy. A lot of drugs can ameliorate the previous consequences like valproate sodium. Pioglitazone is an anti-diabetic drug from thiazolidinediones (TZDs) family which acts as a ligand for the transcription factor, peroxisome proliferator-activated receptor gamma (PPAR-?). It has a wide spectrum of actions including modulation of glucose and lipid homeostasis, inflammation, atherosclerosis, bone remodeling, and cell proliferation. Wistar male rat pups were used as a model of febrile-induced seizure. The pups were injected with lipopolysaccharide (LPS) serotype of Escherichia coli O26:B6 (200 ?g/kg, i.p.) and sub-convulsive dose of kainic acid (KA) (1.75 mg/kg, i.p.) for induction of experimental febrile seizures. After that, anti-inflammatory, neuroprotective, and anti-apoptotic effects of 3 different doses of pioglitazone (5, 10, 20 mg/kg, i.p.) on experimental rat pups were assessed by measurement of interleukin-1 beta (IL-1?), tumor necrosis factor-alpha (TNF-?), inducible nitric oxide synthase (iNOS) levels and apoptosis detection (toluidine blue staining and terminal deoxynucleotidyl transferase (TdT)- mediated dUTP-biotin nick end labeling (TUNEL) assay) in the hippocampus tissues, respectively. Finally, we concluded that pioglitazone exerts anticonvulsant activity in febrile-induced seizure via modulation of inflammation, induction of constitutive nitric oxide synthase, and preventing apoptosis.

Biography :

E-mail: hussein@mail.um.ac.ir