Journal of Microbial & Biochemical Technology

Phytochemical analysis and antibacterial activities of Jatropha curcas on some clinical isolates

International Conference and Summit on Industrial & Pharmaceutical Microbiology

October 17-18, 2016 Kuala Lumpur, Malaysia

Ibrahim Zainab Yau

Ahmadu Bello University, Nigeria

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

This study investigates the use of Jatropha curcas as part of the medicinal plants used in treating many microbial infections. The lack of scientific backup for the use of some herbal preparations as well as fight against antibiotic resistance indicates a strong need for continuous effort to validate the use of plant material as alternative therapy regimens with similar or higher antibiotic beneficial properties. The present study describes the phytochemical activities and antibiotic properties of Jatropha curcas on clinical isolates of S. aureus, E. coli, P. aeruginosa and Bacillus spp. The phytochemical analysis was carried out on stem bark, root bark and leaves of the plant extract which contains carbohydrates, saponins, flavonoids and tannins among others. The antibacterial potency was initially determined by the agar well diffusion method followed by quantitative evaluation of antibacterial activity by MIC and MBC method. Root extract of Jatropha curcas was observed to be more effective than each of the extracts due to its ability to inhibit all the test bacteria with the highest antibacterial activity with mean inhibition zone diameter ranging from 2.67±0-15.33±0 against S. aureus with ethanolic root and 1.4±0-14.7±0 with aqueous root. The aqueous root extract also recorded mean inhibition zone diameter ranging from 10.0-17.5 against Bacillus spp., and 6.5-10 with ethanolic root. A mean inhibition diameter also ranging from 0-6 with ethanolic root and 0-6.50 with aqueous root were also recorded for P. aeruginosa. The probable chemical compounds that lead to the activity against the test organisms were identified by Gas Chromatography/Mass Spectrometer (GC/MS) analysis where acetic acid, linoleic acid ethyl ester and oleic acid methyl ester, 1,3-propanediol, furan-2-ethyl tetrahydro-5-methoxy-2-methyl, hexadecanoic acid, octadecanoic acid as compounds with most antibacterial activities. This study has proven the effectiveness and efficacy of Jatropha curcas in inhibiting the growth of S. aureus, E. coli, P. aeruginosa and Bacillus spp., pathogenic bacteria of clinical origin.

Biography :

Email: zeeyau78@yahoo.com