Journal of Nanomedicine & Nanotechnology

PGJ nanoparticle associated to acellular human amniotic membrane scaffold: A new system for local anti-inflammatory therapy

Nanotechnology Congress & Expo

August 11-13, 2015 Frankfurt, Germany

Julio Cesar Francisco

Poster-Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

The pathogenesis of chronic inflammatory diseases is multifactorial and includes factors as tissue injuries, metabolic disorder and autoimmune diseases. The 15-deoxy-12,14-PG J2 is known for its anti-inflammatory, antioxidant and immunmodulatory properties. In vivo adhesions between cells and the extracellular matrix play a crucial role in cell differentiation, proliferation, and migration as well as tissue remodeling. Here, we present a simple method to incorporate 15d-PGJ2 nanoparticles in acellular human amniotic membrane scaffold, as potential local anti-inflammatory delivery system. After completely removing the cells on the amniotic membrane with a sodium dodecyl sulphate and mechanical approach, we seeded Vero cells incorporate 15d-PGJ2 nanoparticles on it. The morphology of the Vero cells and nanoparticles was observed by scanning electron microscopy (SEM). The cells cultivated observed by scanning electron microscopy (SEM) presented the incorporation of the nanoparticles smooth surface and spherical shape. Our results indicate that the HAM may be an ideal candidate as a nanoparticule-matrix adhesion substrate to study a new system for local anti-inflammatory therapy.