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Peptide-based self-assembled nanovaccine system targeting Group A Streptococcus infection
5th Asia Pacific Global Summit and Expo on Vaccines & Vaccination
July 27-29, 2015 Brisbane, Australia
Guangzu Zhao, Sharareh Eskandari, Mariusz Skwarczynski and Istvan Toth
Posters-Accepted Abstracts: J Vaccines Vaccin
Abstract:
The use of traditional vaccines based on whole microorganisms is often associated with the serious drawbacks such as allergic responses or difficulties in pathogen cultivation. These obstacles can be overcome by peptide-based vaccines. However, as peptide itself has almost no immunogenicity, adjuvant that could evoke and enhance the immune response against a supplied antigen is necessary for peptide subunit vaccine. Currently, the high toxicity and low immunogenicity of current existed adjuvants are the biggest obstacle blocked the application of peptide subunit vaccine. In this project, a novel self-adjuvanting system for peptide subunit vaccine was proposed. This system applied hydrophobic amino acids sequence to which was expected to induce self-assembly of vaccine candidates into nanoparticles. As nanoparticles are known to elicit immune response, this system should find application in vaccine development. GAS B-cell epitope J14 was used in this project to evaluate the efficiency of this system, and T-helper epitope PADRE was also applied. J14, PADRE and poly phenylalanine sequence were linked covalently through the lysine moiety to product the vaccine candidates. Two approaches were attempted to obtain the desired compound. The segment condensation based synthesis approach was unsuccessful due to poor solubility of poly phenylalanine unit in examined solvents. Stepwise synthesis approach was conducted afterwards. The whole desired compounds were synthesized successfully. Formation nanoparticles (10-70nm) were confirmed by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The efficacy of particles to induce immune responses will be analysed in mice model in near future.