Clinical Microbiology: Open Access

PCR detection of bacteriocin genes from lactic acid bacteria isolated from milk

8^thGlobal Summit on Microbiology and Infectious Diseases

February 22-23, 2018 | Paris, France

Nwanekwu K E N

Federal University of Technology Owerri, Nigeria

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

The bacteriocin structural gene in six bacteriocinogenic lactic acid bacteria (LAB) namely Lactobacillus acidophilus, Lactobacillus casei, Streptococcus thermophilus, Lactococcus lactis, Lactobacillus fermentum and Lactobacillus brevis isolated from raw and processed milk samples was studied. Four primers were designed to amplify bacteriocin genes of bacteriocin producing LAB. The primer sets were able to amplify the bacteriocin gene from all the six LAB isolates. Primer A(IcIA) amplified bacteriocin gene of L. casei, L. lactis and L. fermentum, Primer B (acdT) amplified bacteriocin genes of L. acidophilus, L. casei, L. lactis and L. brevis, Primer C (acdA) amplified bacteriocin genes of L. acidophilus, S. thermophilus and L. brevis, while Primer D (Iaf) amplified bacteriocin genes of L. casei and L. brevis. The bacteriocin genes of the organisms were between the ranges of 210-300 bp, which was determined based on the molecular weight marker in gel system. Hybridization analysis confirms the amplicon of this LAB are bacteriocins.