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Parameter validation of analytical methods of insecticide residue analyses in foods of animal origin, feed and water
3rd World Congress Bioavailability & Bioequivalence
March 26-28, 2012 Marriott Hotel & Convention Centre, Hyderabad, India
Amsalu Debebe
Scientific Tracks Abstracts: J Bioequiv Availab
Abstract:
The study was conducted to examine the interrelationship and coherence of analytical parameters in method validation. Recovery, sensitivity, linearity, precision and limits of detection (LOD) were tested in six methods for organochlorine and organophosphate insecticides. Compounds that fell out of the stipulated recovery, 70?120%, in a matrix have concurrently failed to meet the requirements for sensitivity (C0.7), linearity (R2 0.99) and precision (\0.2) in the same matrix. Highest LOD was recorded in those compounds and matrices. Different from the conventional point estimate, a new approach was introduced for setting upper and lower confidence limits of the LOD in quantitative analyses.