Agrotechnology

Pantoea ananatis causing grain discoloration is widely distributed in the rice-growing fields of the Republic of Korea

10^th International Conference on AGRICULTURE & HORTICULTURE

October 02-04, 2017 London, UK

Kim Sang -Min, Ra Ji-Eun, Seo Su Jwa, Lee Bong Choon, and Choi Nak Jung

National Institute of Crop Science, Republic of Korea

Posters & Accepted Abstracts: Agrotechnology

Abstract:

We collected local isolates of Pantoea ananatis causing bacterial grain discoloration from southern rice-growing fields in Korea and constructed phylogenetic tree to see the genetic variation of P. ananatis. In 2016, rice panicles showing symptoms of grain discoloration and grain abortion were collected and the inside of lemma and palea were streaked on the Kingâ??s B agar plate for pathogen isolation. 63 regional isolates were randomly selected for avoiding selection bias and gDNA were extracted for gene-based species identification. Based on 16S rRNA gene, B. glumae, the major pathogen of grain rot, amounted for 49.2% and Pantoea spp. amounted for 22.2%, separately. Multilocus sequence analysis (MLSA) by four gyrB, rpoB, infB, and atpD identified Pantoea spp. as P. ananatis. The pathogenicity of collected P. ananatis isolators were tested and showed weak symptoms of stem rot by artificial injection and weak symptoms of grain discoloration by spraying to the panicles of rice. In this study, we show the incidence and the regional distribution of P. ananatis, and the phylogenetic relationship among P. ananatis regional isolates in Korea, 2016. As the risk of the grain rot and grain discoloration is increasing according to the climatic change, it would be the basic data for understanding P. ananatis in the Republic of Korea.

Biography :

He is a researcher for plant pathology in the National Institute of Crop Science, RDA. Main works include diagnosis, identification and control of crop diseases on the field and on the lab.