Journal of Data Mining in Genomics & Proteomics

Over-expression of c/ebpα gene in bovine muscle stem cell stimulates adipogenesis

2^nd International Conference on Big Data Analysis and Data Mining

November 30-December 01, 2015 San Antonio, USA

Camus Adoligbe, Yifan Huangfu, Linsen Zan and Hong Wang

Northwest A&F University, China
National Beef Cattle Improvement of Northwest A&F University, China

Posters-Accepted Abstracts: J Data Mining Genomics Proteomics

Abstract:

The intramuscular fats (IMF) or Intramuscular triglycerides (IMTG) are present throughout the skeletal muscles. The IMF adds marbling, which is one of the critical criteria for judging the quality of meat cuts, and recently increased IMF in meat has become a focus particularly in the beef industry. The role of CCAAT/enhancer binding protein alpha (C/EBPα) gene in initiating the adipogenic program by over-expressing C/EBPα in bovine muscle stem cells (MSC) has been investigated in the present study in order to provide deep insights, that in-turn could be used to ameliorate beef quality by genetic means. Prior to this, bovine MSC were isolated and induction of their differentiation into adipocyte was tested in cells which were exposed to dexamethasone IBMX and indomethacin in the presence of insulin and fetal bovine serum. Either ectopic expression of C/EBPα or treatment with dexamethasone and insulin is capable of inducing accumulation of fat droplets and expression of adipogenic inducers genes (LPL, PPARγ, C/EBPβ, C/EBPδ). The myoblast related genes (MyoD, Myf5, and Pax7) expressions were also measured to assess the accuracy of the differentiation process. This work has provided evidence that C/EBPα gene is essential for cattle adipose tissue growth and development, information that could be used to improve beef quality.

Biography :

Camus Adoligbe is a progressive and result-oriented person with major interest in the field of animal science focusing on scientific goals and development, community empowerment and Public-Private sector partnership through value chain analysis of scientific processes in order to eliminate inefficiencies. He got his Bachelor degree in Animal production from Abomey-Calavi University (Benin) and his Master degree in Animal Genetics and Breeding from Northwest A&F University (China). He is currently pursuing his Ph.D. in the same field. He is a promising scientist in the field of animal science to lay sound, practical and viable solutions through consultative approach. As per his publication status, he has already published 3 papers and he has others under review.