Journal of Plant Pathology & Microbiology

Outbreak, pathogenicity and biological control of Phytophthora capsici in tomato

2^nd International Conference on Plant Science & Physiology

June 26-27, 2017 Bangkok, Thailand

Ibrahim E Elshahawy, Hesham M Abouelnasr and Sirag M Lashin

National Research Centre, Egypt

Posters & Accepted Abstracts: J Plant Pathol Microbiol

Abstract:

Statement of the Problem: Tomato transplants (30 days old) were grown in fields at Fayoum and Beni Suef governorates in Egypt. Developed symptoms of wilting with brown-to-black cankers on the lower stems and root rot were observed. However, this problem has not been previously studied in Egypt. The purpose of this research is to study the outbreak of this disease, pathogenicity tests of the isolated fungi, identification of the causal agent and the possibility of different biocontrol agents to control the disease under in vitro and greenhouse conditions. Methodology: A survey was conducted in August of 2016 on the outbreak of these symptoms at Fayoum and Beni Suef governorates in Northern Upper Egypt. Pathogens were isolated from infected parts of the transplants by culturing the affected tissues on V8 medium. Pathogenicity tests were conducted on healthy tomato seedlings which were grown under greenhouse conditions. Monitor potential of biocontrol agents against the pathogen were conducted in vitro and under greenhouse conditions. Findings: The diseased plants having the above symptoms were prevalent and severe losses in established plants were found (near 50%) in both governorates. Sixteen fungal isolates belonging to five genera were isolated from affected tissues. Phytophthora capsici recorded the highest frequency of occurrence and was found to be prevalent in most cases. Pathogenicity tests were performed and the fungus was successfully re-isolated from symptomatic plants, fulfilling Kochâ??s postulates. Identification of Phytophthora capsici (Leonian) was based on cultural, morphological and molecular approaches. The latter usage of the primer DC 6 soil drench with the selected strains belonging to the genus Bacillus, Pseudomonas and Trichoderma, significantly reduced disease severity and increased the growth of tomato plants compared to untreated controls in greenhouse experiments. C onclusion & Significance: To the best of our knowledge, this is the first report of phytophthora blight by P. capsici from Egypt. The results indicate that certain Bacillus, Pseudomonas and Trichoderma strains are effective against P. capsici on tomato, and improved plant growth.

Biography :

Email: ibrahim_nrc@yahoo.com