Oral Health and Dental Management

Osteoclastogenic and inflammatory activation of lipoteichoic acid of Fillifactor alocis as a chronic peridontitis related pathogen

32^nd Asia Pacific Dental and Oral Health Congress

July 23- 24, 2018 Sydney, Australia

Sung-Hoon Lee

Dankook University, Republic of Korea

Posters & Accepted Abstracts: Oral Health Dent Manag

Abstract:

Filifactor alocis was recently detected in chronic peridontitis patients and is the only Gram-positive bacterium in the gingival pocket. Lipoteichoic Acid (LTA) is known to be a immune stimulater. The purpose of this study was investigated the effects of LTA of Filifactor alocis on chronic peridotitis realted factors. Filifactor alocis ATCC 35896 was used in this study and cultivated columbia broth including arginine and cysteine. LTA from Filifactor alocis was extracted by butanol extraction followed by HPLC. The extracted LTA was validated with CHO/CD14/TLR2 and CHO/CD14/TLR4 cell. Human gingival fibroblast were treated with the LTA or Lipopolysaccharide (LPS) from Porphyoromonas gingivalis, Tannerella forsythia, and osteoprotegerin (OPG, a osteoclastogenesis inhibitory factor), RNAKL (osteoclastogenesis activation factor) and inflammatory cytokines were analyzed by real-time RT-PCR and ELISA after purifying RNA and collecting conditioned media. The LTA stimulated CHO/CD14/TLR2 cells but did not CHO/CD14/TLR4. The LTA induced higher levels of TNF-α, IL-1β, and IL-6 than LPS from Porphyoromonas gingivalis and Tannerella forsythia. Forthermore, OPG/RANKL ratio were decreased more by LTA from Filifactor alocis than by the LPS. In the conclusion, LTA of Filifactor alocis may be more related with chronic periontitis than the virulence factor of other periodontopathogens.