Journal of Blood Disorders & Transfusion

Osmotic fragility of human erythrocytes in vitro using Vipera lebetina venom

2^nd International Conference on Hematology & Blood Disorders

September 29-October 01, 2014 DoubleTree by Hilton Baltimore-BWI Airport, USA

Nayef S Gharaibeh, Mukhallad A Mohammad, Khalid Talafih, Osama Y Abo Al-Rob and Mohammed Ayesh

Scientific Tracks Abstracts: J Blood Disorders Transf

Abstract:

The objective of this study was to investigate the in vitro effect of Vipera lebetina venom on human erythrocytes? osmotic fragility with respect to different venom concentrations, time of incubation with the venom, initialvolume of red blood cells, and presence of anti-venom. In vitro osmotic fragility of human erythrocytes was determined using Dacie and Lewis method. Osmotic fragility is increased significantly after incubation with the venom atdifferent concentrations. The maximal effect was detected at the venom concentration of 400 μg. Time of incubation with the venom was an important factor in hemolytic process while the initial cell volume has no significant effect. Incubation of envenomed erythrocytes for 120 min with anti-venom did not reverse the changes in osmotic fragilityinduced by the venom. The study suggests that the venom could alter the susceptibility of erythrocytes to hemolysis when subjected to osmotic stress and the degree of hemolysis depends on venom concentration and time of incubation with the venom.