Pharmaceutica Analytica Acta

Optimizations of oxytetracycline residues detection method in milk and study of its clinical elimination

International Conference and Exhibition on Advances in HPLC & Chromatography Techniques

March 14-15, 2016 London, UK

Yuan Liu

China Agricultural University, China

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Here an improved HPLC-PAD method was developed and validated suitably for the residue detection of oxytetracycline in milk. In our study, 0.1 mol/L Na2EDTA - Mcllvaine buffer solution was used to extract oxytetracycline from milk, n-hexane for degreasing, and a 3 CC Oasis HLB solid-phase extraction column for purification. A reversed phase chromatographic column Symmetry Shield RP C18 (250 mmÃ?4.6 mm) with acetonitrile and 0.048% phosphoric acid solution (pH 2.5) as mobile phase by gradient eluting was applied to as the chromatographic separation condition. The results showed that the retention time of oxytetracycline was 6.5 min with a symmetrical and sharp peak, which was well separated from the impurity in the milk sample. Oxytetracycline standard solutions within the concentration range of 5 - 2000 μg/kg, had a good linearity between peak areas and the concentrations, with R2>0.9999. The recoveries of oxytetracycline at three levels of 10, 20, 100 μg/kg were 87.9%, 90.5% and 87.8%, respectively, with the coefficient of variation ranged from 2.2 â?? 5.8% in the day and 4.0 â?? 5.1% between the days. The LOD is 5 μg/kg and the LOQ is 10 μg/kg, which was sensitive enough for residue detection. In conclusion, a simple and reliable method for detection of oxytetracycline in milk was developed and validated. Additionally, the validated method was rapid and sensitive as well as provided high recoveries in milk.

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