Agrotechnology

Optimization of supercritical extraction CO2 of essential oil from neem leaves using response surface methodology

7^th Global Summit on Agriculture & Horticulture

October 17-19, 2016 Kuala Lumpur, Malaysia

Suzana Yusup, Lee Pei Ting, Rohani Salleh, Haryanni Harun, Puteri Sarah Diba Kamarulzaman, Raudah Talib and Sulaiman Mokhtar

University of Technology, Malaysia

Posters & Accepted Abstracts: Agrotechnol

Abstract:

Neem tree or Azadirachta indica has been proven to give a lot of benefits in medicinal point of view. Each parts of the tree have its own biological activity which could aid in fighting and curing diseases. These biological activities were found to come from the sesquiterpenes compounds that exist in the leaf. The main biological activities of neem leaf are antiinflammatory and anti-allergy properties. Supercritical carbon dioxide extraction was applied for the extraction of essential oil since it gives higher oil yield and does not require extra unit to separate the solvent used. The optimization of supercritical carbon dioxide extraction was studied and the optimized parameters were 20.25 MPa pressure and 319.33 K using 0.23 mm sample determined through RSM. GCMS analysis was carried out on the essential oil of neem leaves and 43 compounds were identified. 1.06% of sesquiterpenes compound of γ-elemene and α-farenesene is found. The major compounds in the extract composed mainly carboxylic acids which was 49.11%. 17.02% of hexadecanoic acid, 31.63% of phytol and 24.2% of 9,12,15-octadecatrienoic acid, (Z,Z,Z) were also found in the extract. In addition, supercritical carbon dioxide extraction of essential oil from neem leaves has been proven to give higher oil yield (0.6%) compared to hydrodistillation technique which can only extracted about 0.1% of the essential oil. Supercritical CO2 extraction uses environmental friendly solvent such as CO2 and which is recyclable.

Biography :

Email: drsuzana_yusuf@petronas.com.my