Journal of Bacteriology & Parasitology

Octodenes isolated from the seaweed Octhodes secundiramea for the treatment and control of schistosomiasis

2^nd World Congress on Parasitology, Microbiology and Infection Control

November 08-09, 2021 | Webinar

Sara Guibunda Taju

Universidade Pungue, Manica, Mocambique

Scientific Tracks Abstracts: J Bacteriol Parasitol

Abstract:

Three analogous halogenated monoterpenes were isolated; specifically, three octodenes from the crude dichloromethane extract of the seaweed O. secundiramea: octodene 1 (4-bromo-1,6,8-trichloro-2-3-octodene, C10H14BrCl3) octodene 2 (2-chloro-1,6 , 8-tribromo-3-8-octodene, C10H14Br3Cl) and octodene 3 (1chloro-5,7- dibromo-2-3-octodene, C10H15Br2Cl). These compounds were tested in snail embryos (intermediate host of S. mansoni), in cercariae (S. mansoni larvae), in schistosomules (young S. mansoni parasites) and in adult and mated S. mansoni parasites. Octodenes 1 and 3 killed 100% of snail embryos at the lowest concentration evaluated (6.25 μg / mL). Octodene 2, at the highest concentration evaluated (50 μg / mL) killed 54% of exposed embryos. Regarding the cercarcidal activity, octodenes 1 and 3 killed 100% of the cercariae in the first 5 minutes of the analysis, and octodene 2 in 120 minutes. As for activity in schistosomules, octodenes 1 and 2 caused deaths with IC50 values of 15 and 18 μM, respectively, on the fourth day of treatment. The IC50 of octodene 3 was 158 μM on the fourth day of treatment. Octodene 1 showed activity in adult S. mansoni parasites with an IC50 of 49 μM in males and 53 μM in female; completely inhibited oviposition and showed low cytotoxicity in normal mammalian cells - the IC50 was 450 μM within 24 hours of exposure. Octodene 1 showed to be a promising candidate for combating schistosomiasis as it acts in almost all disease control targets, both in water as molluscicide and cercaricide, as well an anthelmintic acting in young and adult S. mansoni parasites.

Biography :

Sara Guibunda Tajú completed her PhD at the age of 32 at the University of São Paulo and Postdoctoral studies at Instituto Butantan. She is the director of the Faculty of Agricultural and Biological Sciences, an academic unit of the Púnguè University – Mozambique. Professor and researcher in the Biology course at the same faculty. Published articles in renowned journals, book and patent. It has participated and organized several scientific events, from conferences, workshops, seminars, scientific meetings, forums, etc. Principal researcher in the project on “COVID-19” financed by the FNI (National Research Fund). Coordinator of the Undergraduate Course Curriculum Preparation Committee. Member of the committee for the preparation of bases and guidelines for postgraduate courses. Member of the Board of External Evaluation of Curriculum, by CNAQ (National Council for Quality Evaluation).